Copyright © 2005 Cascade BioScience, Inc. All rights reserved.
| Description | Catalog # | Vial Size | Price (USD) |
| Anti-Human BARD1 (exon 4), polyclonal | ABP-6056 | 100 ug | $295 |
|
Immunoprecipitation:
|
use 1 to 4 ug/100 ug cell lysate |
|
Western Blotting:
|
dilute 1:1,000 - 1:5,000 |
|
|
|
Reactivity:
|
human; other species not tested |
|
Specificity:
|
recognizes BARD1 at ~85 kDa in western blots of HeLa cell nuclear extracts; mouse BARD1 shares 21 of 27 amino acid residues within the immunizing sequence |
|
Immunogen:
|
synthetic peptide corresponding to a portion of human BRCA1 associated Ring Domain 1 encoded within exon 4 (LocusLink ID 580) |
|
Host/Ig Type:
|
rabbit polyclonal IgG |
|
Purification:
|
affinity purified using the immunizing peptide immobilized on solid support |
|
Quality Control:
|
by Western blotting (see "Specificity") |
|
Liquid Carrier:
|
1 mg/ml antibody in Tris-citrate/phosphate buffer, pH 7 to 8; 0.1% sodium azide |
|
Storage:
|
4˚C |
|
Stability:
|
1 year |
Western Blot and IP-Western: A. Nuclear extracts from HeLa cells or U2OS cells snf B. U2OS cells expressing endogenous (-) or overexpressed (+) BARD1 probed with Anti-Human BARD1 (exon 4), polyclonal (Cat. #ABM-6056) C. Immunoprecipitation of nuclear extract from HeLa cells or control using normal rabbit IgG and susequently visualized with the antibody