Copyright © 2005 Cascade BioScience, Inc. All rights reserved.
Description | Catalog # | Vial Size | Price (USD) |
Anti-Human Phospho-BRCA1 (pSer1387), polyclonal | ABP-6060 | 100 ug | $295 |
Immunoprecipitation:
|
use 2/5 ug/mg cell lysate |
Western Blotting:
|
dilute 1:1,000 - 1:5,000 |
|
Reactivity:
|
human; other species not tested |
Specificity:
|
recognizes pBRCA1 in western blots of HeLa cell nuclear extracts |
Immunogen:
|
phosphorylated synthetic peptide (pSer1387) corresponding to a portion of human Breast Cancer Gene 1 |
Host/Ig Type:
|
rabbit polyclonal IgG |
Purification:
|
antibodies that were not phospho-specific were removed by solid phase adsorption; specific antibodies were affinity purified using the immunizing phospho-peptide immobilized on solid support |
Quality Control:
|
by Western blotting (see "Specificity") |
Liquid Carrier:
|
1 mg/ml antibody in Tris-citrate/phosphate buffer, pH 7 to 8; 0.1% sodium azide |
Storage:
|
4˚C |
Stability:
|
1 year |
Western Blot: 25 ug nuclear extracts from control (-) and (+) irradiated (10 Gy IR) HeLa cells probed with Anti-Human phospho BRCA1 (pSer1387), polyclonal (Cat. #ABM-6060). 1:3,000 diluion