ABM-5525

Reactivity:	rat, mouse; others likely

Immunogen:	full-length adenovirus vector injected into CD36 null mice
Host/Ig Type:	mouse monoclonal IgA
Purification:	protein L chromatography
Quality Control:	Flow Cytometry using elicited mouse peritoneal macrophages incubated with CD36 antibody

Storage:	-20˚C
Stability:	1 year

Immunofluorescence:
Murine insulin treated or untreated adipocytes fixed with acetone were incubated with murine anti-mouse CD36 antibody (1:100 dilution of supernatent), followed by FITC-conjugated IgA-specific secondary antibody. Confocal microscopy revealed prominent membrane staining (left panels). Phase contrast images are shown in the right panels.

F l ow Cytometry:
Elicited mouse peritoneal macrophages were incubated with murine anti-mouse CD36 antibody (1:100 dilution of supernatent) or an isotype control (IgA), followed by FITC -conjugated IgA specific secondary antibody, and then analyzed by flow cytometry. The shaded peak shows high level expression of CD36.

Febbraio, M., Hajjar, D.P., and R.L. Silverstein. (2001) "CD36: A Class B Scavenger Receptor involved in angiogenesis, atherosclerosis, inflammation and lipid metabolism." J Clin Invest. 2001 Sep;108(6):785-91

Dawson, D.W., Pearce, S.F.A., Zhong R., Silverstein, R.L., Frazier,W.A., and N.P. Bouck. (1997) "CD36 Mediates the Inhibitory Effects of Thrombospondin-1 on Endothelial Cells." J. Cell Biology 138: 707-717

Febbraio, M., Abumrad, N.A., Hajjar, D.P., Sharma, K., Cheng W., Pearce, S.F.A., and R.L. Silverstein. (1999) "A null mutation in murine CD36 reveals an inportant role in fatty acid and lipoprotein metabolism." J. Biol. Chem 274:19055-19062

Silverstein, R. L. and M. Febbraio. (2000) "CD36 and Atherosclerosis. Current Opinions in Lipidology." 11:483-491

Functional Studies:
Antibody blocked uptake of oxidized LDL by murine macrophages and prevented foam cell formation in vitro. Antibody blocked oxidized LDLdependent activation of macrophage JNK kinase.

Chien-Ping Liang, Seongah Han, Haruka Okamoto, Ronald Carnemolla, Ira Tabas, Domenico Accili, and Alan R. Tall “Increased CD36 protein as a response to defective insulin signaling in macrophages” J Clin Invest. 2004 March 1; 113 (5): 764–773
full text

Lynn Corcoran, David Vremec, Maria Febbraio, Tracey Baldwin and Emanuela Handman “Differential regulation of CD36 expression in antigen-presenting cells: Oct-2 dependence in B lymphocytes but not dendritic cells or macrophages” International Immunology, Vol. 14, No. 10, pp. 1099-1104, October 2002
full text

Kuniyasu A, Ohgami N, Hayashi S, Miyazaki A, Horiuchi S, Nakayama H.”CD36-mediated endocytic uptake of advanced glycation end products (AGE) in mouse 3T3-L1 and human subcutaneous adipocytes.” FEBS Lett. 2003 Feb 27;537(1-3):85-90.
Abstract 6 more...

Description	Catalog #	Vial Size	Price (USD)
Anti-Murine CD36 (clone 63)	ABM-5525	100 ug	$295


Immunoprecipitation:	1:1000 dilution
Flow Cytometry	1:1000 dilution
Immunofluorescence:	1:1000 dilution
Funtional Studies	recommended