ABM-2052

Description	Catalog #	Vial Size	Price (USD)
Anti-Human PTEN (clone 6H2.1)	ABM-2052	100 ug	$379

Research Applications

Antibody Characteristics

Western Immunblotting:	1-5 micrograms/ml
Immunoprecipitation:	5 micrograms
Immunocytochemistry:	recommended, protocol
Immunofluorescence:	recommended
ELISA:	recommended

Reactivity:	human, mouse; others likely
Specificity:	single band specific; detects PTEN at ~60 kDa in total cell lysates
Immunogen:	full-length fusion protein; human sequence; epitope in red
Host/Ig Type:	mouse monoclonal IgG
Purification:	protein A chromatography
Quality Control:	by WB on numerous breast cancer cell lines
Liquid Carrier:	phosphate-bufferd saline
Storage:	-20˚C
Stability:	2 years

Immunohistochemical Analysis
Photomicrographs illustrate PTEN non- expressing neoplastic endometrial glands in a background of normal expressing glands and stroma.

Immunoblot Analysis
6H2.1 single band specificity on 7 breast cancer cell lines; one of which is PTEN-null; one of which has a monoallelic PTEN deletion and a missense mutation

Applications References

Ginn-Pease, M.E. and Eng, C., "Increased Nuclear Phosphatase and Tensin Homologue Deleted on Chromosome 10 Is Associated with G0-G1 in MCF-7 Cells" (2003), Cancer Research 63:282-286.
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Schondorf, T., Hoopman, M., Eversheim, B., Valter, M.M., Becker, M., Wappenschmidt, B., Gohring, U.-J., Kubler, T., Schmutzler R. K., and Schafer, R. "Expression of the tumor suppressor gene PTEN is not
altered in the progression of ovarian carcinomas and does not correlate with p27Kip1 expression" (2003) Oncology Reports 10:1717-1722.
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Joëlle Dupont, Jean Pierre Renou, Moshe Shani, Lothar Hennighausen, and Derek LeRoith “PTEN overexpression suppresses proliferation and differentiation and enhances apoptosis of the mouse mammary epithelium” J. Clin. Invest. 110:815–825 (2002).
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