ABM-5525

Description	Catalog #	Vial Size	Price (USD)
Anti-Human Toll-like Receptor 2 (TLR2), clone TL2.1	ABM-5525	100 ug	$295

Research Applications

Antibody Characteristics


Immunoprecipitation:	recommended
Flow Cytometry	recommended
Immunofluorescence:	recommended
Funtional Studies	recommended

Reactivity:	human

Immunogen:	CHO cells transfected with human Toll-like Receptor 2
Host/Ig Type:	mouse monoclonal IgA
Purification:	protein G chromatography
Quality Control:	by Immunoprecipitation of TLR2 from human cell types includieng T-, B- and NK cells
Liquid Carrier:	phosphate-buffered saline
Storage:	-20˚C
Stability:	1 year

About TLR

Immunofluorescence:
Confocal expression in monocyte- macrophages. Day stained with (D) and anti- mAbs. Images (A) Close- macrophage nuclear TLR2 A488).

InfoSheet PDF

F l ow Cytometry:
Expression of TLR2 in human blood cell populations. Whole blood was doublestained with control mAbs (muIgG-A488 and muIgG1 -PE) or mAbs to TLR2 (TL2.1- A488) together with PE-conjugated anti-CD3 and CD14 mAbs and examined by flow cytometry. Contour plots shown are from one representative experiment repeated five times with different donors.

Immunoprecipitation:
IP of TLR2 from human monocytes and T-, B-, and NK cells. 35 S-labeled lysates from purified monocytes and T-, B-, and NK cells untreated (2) or incubated with PMA/ionomycin (1) for 16 h were immuno- precipitated with anti-TLR2 mAb (TL2.1), subjected to gel electrophoresis, and analyzed by autoradiography. Bands from the molecular weight marker (kDa) are indicated.

enlarge image