Copyright © 2004 Cascade BioScience, Inc. All rights reserved.
| Description | Catalog # | Vial Size | Price (USD) |
| Anti-Human AKT1/PKB (paired 473), polyclonal | ABP-6006 | 100 ug | $295 |
|
Western Blot:
|
0.5 -1.0 micrograms/ml |
|
Immunoprecipitation:
|
3-5 micrograms/sample derived from 1 x 10E6 cells |
|
ELISA:
|
0.1-1 micrograms/ml |
|
Reactivity:
|
human, rat, mouse |
|
Specificity:
|
reacts predominantly with AKT1; detects a ~60 kDa immunoreactive protein from different cell lysates and normal mouse/rat tissues in Westen immunoblots; band is abolished by the depletion of the synthetic peptide; samples are boiled prior to SDS-PAGE |
|
Immunogen:
|
synthetic peptide corresponding to the carboxyl terminus of AKT1 surrounding Ser473 from human/rat/mouse origins |
|
Host/Ig Type:
|
rabbit polyclonal IgG |
|
Purification:
|
peptide affinity purification |
|
Quality Control:
|
by western blot (see "Specificity") |
|
Liquid Carrier:
|
phosphate buffered saline (pH 7.4) containing 0.02% Proclin300. |
|
Storage:
|
-20˚C |
|
Stability:
|
2 years |
1. Chan T et al (1999) Annu. Rev. Biochem. 68, 965-1014.
2. Cho H et al (2001) J. Biol. Chem, 276: 38349-38352.
3. Jones PF et al, (1991) Proc. Natl. Acad. Sci. U.S.A. 88: 4171.
4. Cross DA et al (1995) Nature 378: 758.
5. Andjelkovic M et al (1996) Proc. Natl. Acad. Sci. U.S.A 93: 5699.
Western immunoblot:
cell extracts derived from brain, heart, muscle, liver, spleen, thymus, testes, kidney and lung repectively probed with Anti-AKT1 (Paired 308; Cat. #ABP-6005)